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Image Search Results
Journal: Chinese Medical Journal
Article Title: Role of Wnt Inhibitory Factor-1 in Inhibition of Bisdemethoxycurcumin Mediated Epithelial-to-Mesenchymal Transition in Highly Metastatic Lung Cancer 95D Cells
doi: 10.4103/0366-6999.156795
Figure Lengend Snippet: Wnt inhibitory factor-1 (WIF-1) overexpression inhibited cell migration and invasion, epithelial-to-mesenchymal transition. (a) Analysis of WIF-1 expression levels in 95C and 95D cells by reverse transcription-polymerase chain reaction (RT-PCR) (left) and western blot (right); (b) The analysis of overexpressed WIF-1 in 95D cells by RT-PCR (left) and by western blot (right); (c) Wound healing assay represented the migration of 95D cells transfected with pIRES2-ZsGreen-WIF-1 or pIRES2-ZsGreen vector (original magnification × 100); (d) Transwell invasion assay with 95D cells transfected with pIRES2-ZsGreen-WIF-1 or pIRES2-ZsGreen vector. Results were representative of three independent experiments. * P < 0.05 ( t =3.819), versus controls; (e) Western blot data of nuclear β-catenin, WIF-1, E-cadherin, vimentin, c-myc, Snail from 95D cells treated with transforming growth factor-β1. GAPDH was a loading control; (f) Western blot data showed the expression of WIF-1, c-myc and Snail proteins in 95D cells transfected with pIRES2-ZsGreen-WIF-1 or pIRES2-ZsGreen vector (original magnification × 200).
Article Snippet: After 2 weeks of selection, 95D cells stably expressing WIF-1 (95D-WIF-1) and control vector pIRES2-ZsGreen (95D-pIRES2-ZsGreen) were collected and used for subsequent experiments.
Techniques: Over Expression, Migration, Expressing, Reverse Transcription Polymerase Chain Reaction, Western Blot, Wound Healing Assay, Transfection, Plasmid Preparation, Transwell Invasion Assay
Journal: Cell Host & Microbe
Article Title: Strong humoral immune responses against SARS-CoV-2 Spike after BNT162b2 mRNA vaccination with a 16-week interval between doses
doi: 10.1016/j.chom.2021.12.004
Figure Lengend Snippet:
Article Snippet: 293T cells were co-transfected with a
Techniques: Staining, Infection, Recombinant, Modification, Lysis, Expressing, Mutagenesis, Transfection, Western Blot, Software, Flow Cytometry